Laboratory cultured faecal inoculum as a substitute for fresh rumen inoculum for in vitro feed evaluation
نویسندگان
چکیده
منابع مشابه
Effect of human faecal inoculum on in vitro fermentation variables.
1. Homogenized and diluted faeces (66.6 g/l) collected from one human source on three different months was incubated with four standard substrates (oat bran, wheat bran, red kidney beans (Phaseolus vulgaris) and guar gum) for 4, 8, 12 and 24 h. 2. Neutral-detergent fibre and organic matter (OM) digestibility measurements and gas production (ml gas/g OM) were influenced by substrate and incubati...
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Biogas productions of cattle manure using rumen fluid inoculums were determined using batch anaerobic digesters at mesophilic temperatures (room and 38.5 C). The aim of this paper was to analyze the influence of rumen fluid contents on biogas yield from cattle manure using fluid rumen inoculums. A series of laboratory experiments using 400 ml biodigester were performed in batch operation mode. ...
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IMPORTANT POINTS The following key points are discussed: • Quality control microbiology tests require controlled levels of inocula and require fresh preparations of cells for those inocula • The concentration of cells in a suspension can be estimated by optical density, but this must be confirmed by plate count • The optical density readings against cell mass are specific to the microorganism s...
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متن کاملPreparing inoculum for susceptibility testing of anaerobes.
We studied six methods for preparation of inoculum to use in susceptibility tests of anaerobic bacteria to determine viable counts of 15 different species of anaerobes. We counted viable bacteria for each method-organism combination. Methods studied included those used for the more routine tests (broth microdilution and broth-disk elution) and for the National Committee for Clinical Laboratory ...
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ژورنال
عنوان ژورنال: African Journal of Agricultural Research
سال: 2012
ISSN: 1991-637X
DOI: 10.5897/ajar12.1634